Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags

Affinity purification of RNA using the ARiBo tag technology currently provides an ideal approach to quickly prepare RNA with 3′ homogeneity. Here, we explored strategies to also ensure 5′ homogeneity of affinity-purified RNAs. First, we systematically investigated the effect of starting nucleotides...

Descripción completa

Detalles Bibliográficos
Autores principales: Salvail-Lacoste, Alix, Di Tomasso, Geneviève, Piette, Benjamin L., Legault, Pascale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2013
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683919/
https://www.ncbi.nlm.nih.gov/pubmed/23657939
http://dx.doi.org/10.1261/rna.037432.112

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683919/
https://www.ncbi.nlm.nih.gov/pubmed/23657939
http://dx.doi.org/10.1261/rna.037432.112

Ejemplares similares