In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR

BACKGROUND: Clinical specimens are routinely fixed in 10% buffered formalin and paraffin embedded. Although DNA is commonly extracted from fixed tissues and amplified by PCR, the effects of formalin fixation are relatively unknown. Formalin fixation is known to impair PCR, presumably through damage...

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Detalles Bibliográficos
Autores principales: Quach, Nancy, Goodman, Myron F, Shibata, Darryl
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC368439/
https://www.ncbi.nlm.nih.gov/pubmed/15028125
http://dx.doi.org/10.1186/1472-6890-4-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC368439/
https://www.ncbi.nlm.nih.gov/pubmed/15028125
http://dx.doi.org/10.1186/1472-6890-4-1

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