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Celastrol Prevents Atherosclerosis via Inhibiting LOX-1 and Oxidative Stress
Celastrol is a triterpenoid compound extracted from the Chinese herb Tripterygium wilfordii Hook F. Previous research has revealed its anti-oxidant, anti-inflammatory, anti-cancer and immunosuppressive properties. Here, we investigated whether celastrol inhibits oxidized low-density lipoprotein (oxL...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684610/ https://www.ncbi.nlm.nih.gov/pubmed/23799016 http://dx.doi.org/10.1371/journal.pone.0065477 |
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author | Gu, Lei Bai, Wenli Li, Sha Zhang, Yuqing Han, Yi Gu, Yue Meng, Guoliang Xie, Liping Wang, Jing Xiao, Yujiao Shan, Liyang Zhou, Suming Wei, Lei Ferro, Albert Ji, Yong |
author_facet | Gu, Lei Bai, Wenli Li, Sha Zhang, Yuqing Han, Yi Gu, Yue Meng, Guoliang Xie, Liping Wang, Jing Xiao, Yujiao Shan, Liyang Zhou, Suming Wei, Lei Ferro, Albert Ji, Yong |
author_sort | Gu, Lei |
collection | PubMed |
description | Celastrol is a triterpenoid compound extracted from the Chinese herb Tripterygium wilfordii Hook F. Previous research has revealed its anti-oxidant, anti-inflammatory, anti-cancer and immunosuppressive properties. Here, we investigated whether celastrol inhibits oxidized low-density lipoprotein (oxLDL) induced oxidative stress in RAW 264.7 cells. In addition, the effect of celastrol on atherosclerosis in vivo was assessed in apolipoprotein E knockout (apoE(−/−)) mouse fed a high-fat/high-cholesterol diet (HFC). We found that celastrol significantly attenuated oxLDL-induced excessive expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) and generation of reactive oxygen species (ROS) in cultured RAW264.7 macrophages. Celastrol also decreased IκB phosphorylation and degradation and reduced production of inducible nitric oxide synthase (iNOS), nitric oxide (NO) and proinflammatory cytokines such as tumor necrosis factor (TNF)-α and IL-6. Celastrol reduced atherosclerotic plaque size in apoE(−/−) mice. The expression of LOX-1 within the atherosclerotic lesions and generation of superoxide in mouse aorta were also significantly reduced by celastrol while the lipid profile was not improved. In conclusion, our results show that celastrol inhibits atherosclerotic plaque developing in apoE(−/−) mice via inhibiting LOX-1 and oxidative stress. |
format | Online Article Text |
id | pubmed-3684610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36846102013-06-24 Celastrol Prevents Atherosclerosis via Inhibiting LOX-1 and Oxidative Stress Gu, Lei Bai, Wenli Li, Sha Zhang, Yuqing Han, Yi Gu, Yue Meng, Guoliang Xie, Liping Wang, Jing Xiao, Yujiao Shan, Liyang Zhou, Suming Wei, Lei Ferro, Albert Ji, Yong PLoS One Research Article Celastrol is a triterpenoid compound extracted from the Chinese herb Tripterygium wilfordii Hook F. Previous research has revealed its anti-oxidant, anti-inflammatory, anti-cancer and immunosuppressive properties. Here, we investigated whether celastrol inhibits oxidized low-density lipoprotein (oxLDL) induced oxidative stress in RAW 264.7 cells. In addition, the effect of celastrol on atherosclerosis in vivo was assessed in apolipoprotein E knockout (apoE(−/−)) mouse fed a high-fat/high-cholesterol diet (HFC). We found that celastrol significantly attenuated oxLDL-induced excessive expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) and generation of reactive oxygen species (ROS) in cultured RAW264.7 macrophages. Celastrol also decreased IκB phosphorylation and degradation and reduced production of inducible nitric oxide synthase (iNOS), nitric oxide (NO) and proinflammatory cytokines such as tumor necrosis factor (TNF)-α and IL-6. Celastrol reduced atherosclerotic plaque size in apoE(−/−) mice. The expression of LOX-1 within the atherosclerotic lesions and generation of superoxide in mouse aorta were also significantly reduced by celastrol while the lipid profile was not improved. In conclusion, our results show that celastrol inhibits atherosclerotic plaque developing in apoE(−/−) mice via inhibiting LOX-1 and oxidative stress. Public Library of Science 2013-06-17 /pmc/articles/PMC3684610/ /pubmed/23799016 http://dx.doi.org/10.1371/journal.pone.0065477 Text en © 2013 Gu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Gu, Lei Bai, Wenli Li, Sha Zhang, Yuqing Han, Yi Gu, Yue Meng, Guoliang Xie, Liping Wang, Jing Xiao, Yujiao Shan, Liyang Zhou, Suming Wei, Lei Ferro, Albert Ji, Yong Celastrol Prevents Atherosclerosis via Inhibiting LOX-1 and Oxidative Stress |
title | Celastrol Prevents Atherosclerosis via Inhibiting LOX-1 and Oxidative Stress |
title_full | Celastrol Prevents Atherosclerosis via Inhibiting LOX-1 and Oxidative Stress |
title_fullStr | Celastrol Prevents Atherosclerosis via Inhibiting LOX-1 and Oxidative Stress |
title_full_unstemmed | Celastrol Prevents Atherosclerosis via Inhibiting LOX-1 and Oxidative Stress |
title_short | Celastrol Prevents Atherosclerosis via Inhibiting LOX-1 and Oxidative Stress |
title_sort | celastrol prevents atherosclerosis via inhibiting lox-1 and oxidative stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684610/ https://www.ncbi.nlm.nih.gov/pubmed/23799016 http://dx.doi.org/10.1371/journal.pone.0065477 |
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