Depletion of polycistronic transcripts using short interfering RNAs: cDNA synthesis method affects levels of non-targeted genes determined by quantitative PCR

BACKGROUND: Short interfering RNAs (siRNAs) are often used to deplete viral polycistronic transcripts, such as those encoded by human papillomavirus (HPV). There are conflicting data in the literature concerning how siRNAs targeting one HPV gene can affect levels of other genes in the polycistronic...

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Detalles Bibliográficos
Autores principales: Hanning, Jennifer E, Groves, Ian J, Pett, Mark R, Coleman, Nicholas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3685557/
https://www.ncbi.nlm.nih.gov/pubmed/23693071
http://dx.doi.org/10.1186/1743-422X-10-159

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3685557/
https://www.ncbi.nlm.nih.gov/pubmed/23693071
http://dx.doi.org/10.1186/1743-422X-10-159

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