Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR

mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly used in protocols for analysis of mRNA stability. Here we show that such trea...

Descripción completa

Detalles Bibliográficos
Autores principales: Kucharova, Veronika, Strand, Trine Aakvik, Almaas, Eivind, Naas, Adrian E., Brautaset, Trygve, Valla, Svein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686738/
https://www.ncbi.nlm.nih.gov/pubmed/23840466
http://dx.doi.org/10.1371/journal.pone.0066429

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686738/
https://www.ncbi.nlm.nih.gov/pubmed/23840466
http://dx.doi.org/10.1371/journal.pone.0066429

Ejemplares similares