Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA

BACKGROUND: In bacterial systems, the sequence congruence of genomic DNA (gDNA) and cDNA obtained following reverse transcription of RNA, makes gDNA an automatic target for qPCR primers. This could lead to aberrant gene expression quantification. This is why a rigorous treatment of bacterial RNA wit...

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Detalles Bibliográficos
Autores principales: Gadkar, Vijay J, Filion, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689636/
https://www.ncbi.nlm.nih.gov/pubmed/23369378
http://dx.doi.org/10.1186/1472-6750-13-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689636/
https://www.ncbi.nlm.nih.gov/pubmed/23369378
http://dx.doi.org/10.1186/1472-6750-13-7

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