KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry

Many candidate unnatural DNA base pairs have been developed, but surprisingly, some of the best replicated adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at differ...

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Autores principales: Betz, Karin, Malyshev, Denis A., Lavergne, Thomas, Welte, Wolfram, Diederichs, Kay, Dwyer, Tammy J., Ordoukhanian, Phillip, Romesberg, Floyd E., Marx, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690913/
https://www.ncbi.nlm.nih.gov/pubmed/22660438
http://dx.doi.org/10.1038/nchembio.966
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author Betz, Karin
Malyshev, Denis A.
Lavergne, Thomas
Welte, Wolfram
Diederichs, Kay
Dwyer, Tammy J.
Ordoukhanian, Phillip
Romesberg, Floyd E.
Marx, Andreas
author_facet Betz, Karin
Malyshev, Denis A.
Lavergne, Thomas
Welte, Wolfram
Diederichs, Kay
Dwyer, Tammy J.
Ordoukhanian, Phillip
Romesberg, Floyd E.
Marx, Andreas
author_sort Betz, Karin
collection PubMed
description Many candidate unnatural DNA base pairs have been developed, but surprisingly, some of the best replicated adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replicating one of the more promising pairs, dNaM-d5SICS, and show that efficient replication results from the polymerase itself inducing the required natural-like structure.
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spelling pubmed-36909132013-06-24 KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry Betz, Karin Malyshev, Denis A. Lavergne, Thomas Welte, Wolfram Diederichs, Kay Dwyer, Tammy J. Ordoukhanian, Phillip Romesberg, Floyd E. Marx, Andreas Nat Chem Biol Article Many candidate unnatural DNA base pairs have been developed, but surprisingly, some of the best replicated adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replicating one of the more promising pairs, dNaM-d5SICS, and show that efficient replication results from the polymerase itself inducing the required natural-like structure. 2012-06-03 2012-07 /pmc/articles/PMC3690913/ /pubmed/22660438 http://dx.doi.org/10.1038/nchembio.966 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Betz, Karin
Malyshev, Denis A.
Lavergne, Thomas
Welte, Wolfram
Diederichs, Kay
Dwyer, Tammy J.
Ordoukhanian, Phillip
Romesberg, Floyd E.
Marx, Andreas
KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
title KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
title_full KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
title_fullStr KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
title_full_unstemmed KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
title_short KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
title_sort klentaq polymerase replicates unnatural base pairs by inducing a watson-crick geometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690913/
https://www.ncbi.nlm.nih.gov/pubmed/22660438
http://dx.doi.org/10.1038/nchembio.966
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