Cargando…

KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry

Many candidate unnatural DNA base pairs have been developed, but surprisingly, some of the best replicated adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at differ...

Descripción completa

Detalles Bibliográficos
Autores principales:	Betz, Karin, Malyshev, Denis A., Lavergne, Thomas, Welte, Wolfram, Diederichs, Kay, Dwyer, Tammy J., Ordoukhanian, Phillip, Romesberg, Floyd E., Marx, Andreas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690913/ https://www.ncbi.nlm.nih.gov/pubmed/22660438 http://dx.doi.org/10.1038/nchembio.966

Ejemplares similares

A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase
por: Wu, Eugene Y., et al.
Publicado: (2014)

Systematic exploration of a class of hydrophobic unnatural base pairs yields multiple new candidates for the expansion of the genetic alphabet
por: Dhami, Kirandeep, et al.
Publicado: (2014)

Crick, Watson y el ADN
por: Strathern, Paul, 1940-
Publicado: (1999)

The multiple personalities of Watson and Crick strands
por: Cartwright, Reed A, et al.
Publicado: (2011)

An innate twist between Crick’s wobble and Watson-Crick base pairs
por: Ananth, Prakash, et al.
Publicado: (2013)

Accurate geometrical restraints for Watson–Crick base pairs
por: Gilski, Miroslaw, et al.
Publicado: (2019)

Non-dissociative structural transitions of the Watson-Crick and reverse Watson-Crick А·Т DNA base pairs into the Hoogsteen and reverse Hoogsteen forms
por: Brovarets’, Ol’ha O., et al.
Publicado: (2018)

Non-Watson–Crick RNA synthesis suited to origin functions
por: Puthenvedu, Deepa, et al.
Publicado: (2018)

Effect of loop length variation on quadruplex-Watson Crick duplex competition
por: Kumar, Niti, et al.
Publicado: (2008)

A rule of seven in Watson-Crick base pairing of mismatched sequences
por: Cisse, Ibrahim I., et al.
Publicado: (2012)

Atomistic insight into the kinetic pathways for Watson–Crick to Hoogsteen transitions in DNA
por: Vreede, Jocelyne, et al.
Publicado: (2019)

Watson–Crick-like pairs in CCUG repeats: evidence for tautomeric shifts or protonation
por: Rypniewski, Wojciech, et al.
Publicado: (2016)

Crystallographic evidence of Watson–Crick connectivity in the base pair of anionic adenine with thymine
por: Mishra, Manish Kumar, et al.
Publicado: (2020)

Substituent effects on the pairing and polymerase recognition of simple unnatural base pairs
por: Hwang, Gil Tae, et al.
Publicado: (2006)

Recognition of Watson-Crick base pairs: constraints and limits due to geometric selection and tautomerism
por: Westhof, Eric, et al.
Publicado: (2014)

Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson–Crick selectivity
por: Smith, Gregory P., et al.
Publicado: (2018)

Multiscale Modeling of Wobble to Watson–Crick-Like Guanine–Uracil Tautomerization Pathways in RNA
por: Chandorkar, Shreya, et al.
Publicado: (2021)

Alchemical Free-Energy Calculations of Watson–Crick and Hoogsteen Base Pairing Interconversion in DNA
por: Geronimo, Inacrist, et al.
Publicado: (2022)

The effects of unnatural base pairs and mispairs on DNA duplex stability and solvation
por: Hwang, Gil Tae, et al.
Publicado: (2009)

Comparable Stability of Hoogsteen and Watson–Crick Base Pairs in Ionic Liquid Choline Dihydrogen Phosphate
por: Tateishi-Karimata, Hisae, et al.
Publicado: (2014)

Comprehensive thermodynamic analysis of 3′ double-nucleotide overhangs neighboring Watson–Crick terminal base pairs
por: O'Toole, Amanda S., et al.
Publicado: (2006)

Characterization of the Trans Watson-Crick GU Base Pair Located in the Catalytic Core of the Antigenomic HDV Ribozyme
por: Lévesque, Dominique, et al.
Publicado: (2012)

Silver (I) as DNA glue: Ag(+)-mediated guanine pairing revealed by removing Watson-Crick constraints
por: Swasey, Steven M., et al.
Publicado: (2015)

Validation of the nearest-neighbor model for Watson–Crick self-complementary DNA duplexes in molecular crowding condition
por: Ghosh, Saptarshi, et al.
Publicado: (2019)

Context-sensitivity of isosteric substitutions of non-Watson–Crick basepairs in recurrent RNA 3D motifs
por: Khisamutdinov, Emil F, et al.
Publicado: (2021)

Watson-Crick Base-Pairing Requirements for ssDNA Recognition and Processing in Replication-Initiating HUH Endonucleases
por: Smiley, Adam T., et al.
Publicado: (2022)

The Role of Electrostatic Interactions on Klentaq1 Insight for Domain Separation
por: Nurbaiti, Santi, et al.
Publicado: (2012)

Higher order structural effects stabilizing the reverse Watson–Crick Guanine-Cytosine base pair in functional RNAs
por: Chawla, Mohit, et al.
Publicado: (2014)

Free energy landscape and transition pathways from Watson–Crick to Hoogsteen base pairing in free duplex DNA
por: Yang, Changwon, et al.
Publicado: (2015)

Selective prebiotic conversion of pyrimidine and purine anhydronucleosides into Watson-Crick base-pairing arabino-furanosyl nucleosides in water
por: Roberts, Samuel J., et al.
Publicado: (2018)

Molecular crowding induces primer extension by RNA polymerase through base stacking beyond Watson–Crick rules
por: Takahashi, Shuntaro, et al.
Publicado: (2020)

Nearest neighbor parameters for Watson–Crick complementary heteroduplexes formed between 2′-O-methyl RNA and RNA oligonucleotides
por: Kierzek, Elzbieta, et al.
Publicado: (2006)

Intermolecular ‘cross-torque’: the N(4)-cytosine propargyl residue is rotated to the ‘CH’-edge as a result of Watson–Crick interaction
por: Domingo, Olwen, et al.
Publicado: (2015)

Probing Watson-Crick and Hoogsteen base pairing in duplex DNA using dynamic nuclear polarization solid-state NMR spectroscopy
por: Conroy, Daniel W., et al.
Publicado: (2022)

Watson‐Crick Base Pairing of N‐Methoxy‐1,3‐Oxazinane (MOANA) Nucleoside Analogues within Double‐Helical DNA
por: Afari, Mark N. K., et al.
Publicado: (2023)

The Structural Basis for Processing of Unnatural Base Pairs by DNA Polymerases
por: Marx, Andreas, et al.
Publicado: (2020)

Thermodynamic stability and Watson–Crick base pairing in the seed duplex are major determinants of the efficiency of the siRNA-based off-target effect
por: Ui-Tei, Kumiko, et al.
Publicado: (2008)

Silver(I) Coordination in Silver(I)‐Mediated Homo Base Pairs of 6‐Pyrazolylpurine in DNA Duplexes Involves the Watson–Crick Edge
por: Escher, Daniela, et al.
Publicado: (2020)

Rapid assessment of Watson–Crick to Hoogsteen exchange in unlabeled DNA duplexes using high-power SELOPE imino (1)H CEST
por: Liu, Bei, et al.
Publicado: (2021)

Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymerase
por: Sakhabutdinova, Assol R., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ufg49e0cl8rgljta81pkcca1er