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Ubiquitin Reference Technique and Its Use in Ubiquitin-Lacking Prokaryotes
In a pulse-chase assay, the in vivo degradation of a protein is measured through a brief labeling of cells with, for example, a radioactive amino acid, followed by cessation of labeling and analysis of cell extracts prepared at different times afterward (“chase”), using immunoprecipitation, electrop...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692480/ https://www.ncbi.nlm.nih.gov/pubmed/23825692 http://dx.doi.org/10.1371/journal.pone.0067952 |