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Sequencing viral genomes from a single isolated plaque

BACKGROUND: Whole genome sequencing of viruses and bacteriophages is often hindered because of the need for large quantities of genomic material. A method is described that combines single plaque sequencing with an optimization of Sequence Independent Single Primer Amplification (SISPA). This method...

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Detalles Bibliográficos
Autores principales: DePew, Jessica, Zhou, Bin, McCorrison, Jamison M, Wentworth, David E, Purushe, Janaki, Koroleva, Galina, Fouts, Derrick E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693891/
https://www.ncbi.nlm.nih.gov/pubmed/23742765
http://dx.doi.org/10.1186/1743-422X-10-181