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Screening and Improving the Recombinant Nitrilases and Application in Biotransformation of Iminodiacetonitrile to Iminodiacetic Acid

In this study, several nitrilase genes from phylogenetically distinct organisms were expressed and purified in E. coli in order to study their ability to mediate the biotransformation of nitriles. We identified three nitrilases: Acidovorax facilis nitrilase (AcN); Alcaligenes fecalis nitrilase (AkN)...

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Detalles Bibliográficos
Autores principales: Liu, Zhi-Qiang, Baker, Peter James, Cheng, Feng, Xue, Ya-Ping, Zheng, Yu-Guo, Shen, Yin-Chu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695085/
https://www.ncbi.nlm.nih.gov/pubmed/23826231
http://dx.doi.org/10.1371/journal.pone.0067197