Enhanced Yield of Recombinant Proteins with Site-Specifically Incorporated Unnatural Amino Acids Using a Cell-Free Expression System

Using a commercial protein expression system, we sought the crucial elements and conditions for the expression of proteins with genetically encoded unnatural amino acids. By identifying the most important translational components, we were able to increase suppression efficiency to 55% and to increas...

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Detalles Bibliográficos
Autores principales: Smolskaya, Sviatlana, Zhang, Zhiwen Jonathan, Alfonta, Lital
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699557/
https://www.ncbi.nlm.nih.gov/pubmed/23844190
http://dx.doi.org/10.1371/journal.pone.0068363

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699557/
https://www.ncbi.nlm.nih.gov/pubmed/23844190
http://dx.doi.org/10.1371/journal.pone.0068363

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