Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids

To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87...

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Detalles Bibliográficos
Autores principales: Zhang, Jun-Hui, Jiang, Yu-Yan, Lin, Ying, Sun, Yu-Fei, Zheng, Sui-Ping, Han, Shuang-Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700896/
https://www.ncbi.nlm.nih.gov/pubmed/23844120
http://dx.doi.org/10.1371/journal.pone.0067892

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700896/
https://www.ncbi.nlm.nih.gov/pubmed/23844120
http://dx.doi.org/10.1371/journal.pone.0067892

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