pK(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography

BACKGROUND: In this study, a RP - HPLC method was applied for determination of pK(a) value by using the dependence of the capacity factor (k) on the pH of the mobile phase for oxysophocarpine (OSP). FINDINGS: The effect of the mobile phase composition on the ionization constant was studied by measur...

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Detalles Bibliográficos
Autores principales: Huo, Huiling, Li, Tingting, Zhang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701144/
https://www.ncbi.nlm.nih.gov/pubmed/23853749
http://dx.doi.org/10.1186/2193-1801-2-270
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author Huo, Huiling
Li, Tingting
Zhang, Lei
author_facet Huo, Huiling
Li, Tingting
Zhang, Lei
author_sort Huo, Huiling
collection PubMed
description BACKGROUND: In this study, a RP - HPLC method was applied for determination of pK(a) value by using the dependence of the capacity factor (k) on the pH of the mobile phase for oxysophocarpine (OSP). FINDINGS: The effect of the mobile phase composition on the ionization constant was studied by measuring the pK(a) value at different MeOH concentrations, ranging from 10 to 20% (v/v). Based on all pH - k curves plotted and pH values at inflection point calculated, experimental pK(a) value obtained for oxysophocarpine was 6.5. CONCLUSION: This method was successfully applied to realize low sample consumption, rapid sample throughput, high sensitivity and precision.
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spelling pubmed-37011442013-07-12 pK(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography Huo, Huiling Li, Tingting Zhang, Lei Springerplus Short Report BACKGROUND: In this study, a RP - HPLC method was applied for determination of pK(a) value by using the dependence of the capacity factor (k) on the pH of the mobile phase for oxysophocarpine (OSP). FINDINGS: The effect of the mobile phase composition on the ionization constant was studied by measuring the pK(a) value at different MeOH concentrations, ranging from 10 to 20% (v/v). Based on all pH - k curves plotted and pH values at inflection point calculated, experimental pK(a) value obtained for oxysophocarpine was 6.5. CONCLUSION: This method was successfully applied to realize low sample consumption, rapid sample throughput, high sensitivity and precision. Springer International Publishing 2013-06-20 /pmc/articles/PMC3701144/ /pubmed/23853749 http://dx.doi.org/10.1186/2193-1801-2-270 Text en © Huo et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Huo, Huiling
Li, Tingting
Zhang, Lei
pK(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography
title pK(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography
title_full pK(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography
title_fullStr pK(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography
title_full_unstemmed pK(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography
title_short pK(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography
title_sort pk(a) determination of oxysophocarpine by reversed - phase high performance liquid chromatography
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701144/
https://www.ncbi.nlm.nih.gov/pubmed/23853749
http://dx.doi.org/10.1186/2193-1801-2-270
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AT zhanglei pkadeterminationofoxysophocarpinebyreversedphasehighperformanceliquidchromatography

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