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Heterologous expression and characterization of a malathion-hydrolyzing carboxylesterase from a thermophilic bacterium, Alicyclobacillus tengchongensis

A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly con...

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Detalles Bibliográficos
Autores principales: Xie, Zhenrong, Xu, Bo, Ding, Junmei, Liu, Lingyun, Zhang, Xuelin, Li, Junjun, Huang, Zunxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701795/
https://www.ncbi.nlm.nih.gov/pubmed/23801110
http://dx.doi.org/10.1007/s10529-013-1195-5