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Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence
We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protei...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701896/ https://www.ncbi.nlm.nih.gov/pubmed/23827883 http://dx.doi.org/10.1038/srep02151 |