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A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs
BACKGROUND: The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate mem...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702409/ https://www.ncbi.nlm.nih.gov/pubmed/23663692 http://dx.doi.org/10.1186/1472-6750-13-41 |