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Accurate and Precise DNA Quantification in the Presence of Different Amplification Efficiencies Using an Improved Cy0 Method

Quantitative real-time PCR represents a highly sensitive and powerful technology for the quantification of DNA. Although real-time PCR is well accepted as the gold standard in nucleic acid quantification, there is a largely unexplored area of experimental conditions that limit the application of the...

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Detalles Bibliográficos
Autores principales: Guescini, Michele, Sisti, Davide, Rocchi, Marco B. L., Panebianco, Renato, Tibollo, Pasquale, Stocchi, Vilberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3704541/
https://www.ncbi.nlm.nih.gov/pubmed/23861909
http://dx.doi.org/10.1371/journal.pone.0068481