Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

BACKGROUND: Metabolic engineering projects often require integration of multiple genes in order to control the desired phenotype. However, this often requires iterative rounds of engineering because many current insertion approaches are limited by the size of the DNA that can be transferred onto the...

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Detalles Bibliográficos
Autores principales: Sabri, Suriana, Steen, Jennifer A, Bongers, Mareike, Nielsen, Lars K, Vickers, Claudia E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706339/
https://www.ncbi.nlm.nih.gov/pubmed/23799955
http://dx.doi.org/10.1186/1475-2859-12-60

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706339/
https://www.ncbi.nlm.nih.gov/pubmed/23799955
http://dx.doi.org/10.1186/1475-2859-12-60

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