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A reliable and feasible qPCR strategy for titrating AAV vectors

BACKGROUND: Previous studies have revealed that traditional real-time quantitative PCR (qPCR) underestimates adeno-associated virus (AAV) titer. Because the inverted terminal repeat (ITR) exists in all AAV vectors, the only remaining element from the wild genome could form special configurations to...

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Detalles Bibliográficos
Autores principales: Wang, Feng, Cui, Xiuling, Wang, Mingxi, Wu, Yaqing, Xiao, Weidong, Xu, Ruian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706409/
https://www.ncbi.nlm.nih.gov/pubmed/23828206
http://dx.doi.org/10.12659/MSMBR.883968