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A Targetron System for Gene Targeting in Thermophiles and Its Application in Clostridium thermocellum
BACKGROUND: Targetrons are gene targeting vectors derived from mobile group II introns. They consist of an autocatalytic intron RNA (a “ribozyme”) and an intron-encoded reverse transcriptase, which use their combined activities to achieve highly efficient site-specific DNA integration with readily p...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706431/ https://www.ncbi.nlm.nih.gov/pubmed/23874856 http://dx.doi.org/10.1371/journal.pone.0069032 |