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Construction of a Full-Length Enriched cDNA Library and Preliminary Analysis of Expressed Sequence Tags from Bengal Tiger Panthera tigris tigris

In this study, a full-length enriched cDNA library was successfully constructed from Bengal tiger, Panthera tigris tigris, the most well-known wild Animal. Total RNA was extracted from cultured Bengal tiger fibroblasts in vitro. The titers of primary and amplified libraries were 1.28 × 10(6) pfu/mL...

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Detalles Bibliográficos
Autores principales: Liu, Changqing, Liu, Dan, Guo, Yu, Lu, Taofeng, Li, Xiangchen, Zhang, Minghai, Ma, Jianzhang, Ma, Yuehui, Guan, Weijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709719/
https://www.ncbi.nlm.nih.gov/pubmed/23708105
http://dx.doi.org/10.3390/ijms140611072