Cargando…

Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses

BACKGROUND: In the intracellular pathogen Brucella spp., the activation of the stringent response, a global regulatory network providing rapid adaptation to growth-affecting stress conditions such as nutrient deficiency, is essential for replication in the host. A single, bi-functional enzyme Rsh ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Hanna, Nabil, Ouahrani-Bettache, Safia, Drake, Kenneth L, Adams, L Garry, Köhler, Stephan, Occhialini, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710219/
https://www.ncbi.nlm.nih.gov/pubmed/23834488
http://dx.doi.org/10.1186/1471-2164-14-459
_version_ 1782276846505689088
author Hanna, Nabil
Ouahrani-Bettache, Safia
Drake, Kenneth L
Adams, L Garry
Köhler, Stephan
Occhialini, Alessandra
author_facet Hanna, Nabil
Ouahrani-Bettache, Safia
Drake, Kenneth L
Adams, L Garry
Köhler, Stephan
Occhialini, Alessandra
author_sort Hanna, Nabil
collection PubMed
description BACKGROUND: In the intracellular pathogen Brucella spp., the activation of the stringent response, a global regulatory network providing rapid adaptation to growth-affecting stress conditions such as nutrient deficiency, is essential for replication in the host. A single, bi-functional enzyme Rsh catalyzes synthesis and hydrolysis of the alarmone (p)ppGpp, responsible for differential gene expression under stringent conditions. RESULTS: cDNA microarray analysis allowed characterization of the transcriptional profiles of the B. suis 1330 wild-type and Δrsh mutant in a minimal medium, partially mimicking the nutrient-poor intramacrophagic environment. A total of 379 genes (11.6% of the genome) were differentially expressed in a rsh-dependent manner, of which 198 were up-, and 181 were down-regulated. The pleiotropic character of the response was confirmed, as the genes encoded an important number of transcriptional regulators, cell envelope proteins, stress factors, transport systems, and energy metabolism proteins. Virulence genes such as narG and sodC, respectively encoding respiratory nitrate reductase and superoxide dismutase, were under the positive control of (p)ppGpp, as well as expression of the cbb3-type cytochrome c oxidase, essential for chronic murine infection. Methionine was the only amino acid whose biosynthesis was absolutely dependent on stringent response in B. suis. CONCLUSIONS: The study illustrated the complexity of the processes involved in adaptation to nutrient starvation, and contributed to a better understanding of the correlation between stringent response and Brucella virulence. Most interestingly, it clearly indicated (p)ppGpp-dependent cross-talk between at least three stress responses playing a central role in Brucella adaptation to the host: nutrient, oxidative, and low-oxygen stress.
format Online
Article
Text
id pubmed-3710219
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-37102192013-07-13 Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses Hanna, Nabil Ouahrani-Bettache, Safia Drake, Kenneth L Adams, L Garry Köhler, Stephan Occhialini, Alessandra BMC Genomics Research Article BACKGROUND: In the intracellular pathogen Brucella spp., the activation of the stringent response, a global regulatory network providing rapid adaptation to growth-affecting stress conditions such as nutrient deficiency, is essential for replication in the host. A single, bi-functional enzyme Rsh catalyzes synthesis and hydrolysis of the alarmone (p)ppGpp, responsible for differential gene expression under stringent conditions. RESULTS: cDNA microarray analysis allowed characterization of the transcriptional profiles of the B. suis 1330 wild-type and Δrsh mutant in a minimal medium, partially mimicking the nutrient-poor intramacrophagic environment. A total of 379 genes (11.6% of the genome) were differentially expressed in a rsh-dependent manner, of which 198 were up-, and 181 were down-regulated. The pleiotropic character of the response was confirmed, as the genes encoded an important number of transcriptional regulators, cell envelope proteins, stress factors, transport systems, and energy metabolism proteins. Virulence genes such as narG and sodC, respectively encoding respiratory nitrate reductase and superoxide dismutase, were under the positive control of (p)ppGpp, as well as expression of the cbb3-type cytochrome c oxidase, essential for chronic murine infection. Methionine was the only amino acid whose biosynthesis was absolutely dependent on stringent response in B. suis. CONCLUSIONS: The study illustrated the complexity of the processes involved in adaptation to nutrient starvation, and contributed to a better understanding of the correlation between stringent response and Brucella virulence. Most interestingly, it clearly indicated (p)ppGpp-dependent cross-talk between at least three stress responses playing a central role in Brucella adaptation to the host: nutrient, oxidative, and low-oxygen stress. BioMed Central 2013-07-08 /pmc/articles/PMC3710219/ /pubmed/23834488 http://dx.doi.org/10.1186/1471-2164-14-459 Text en Copyright © 2013 Hanna et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hanna, Nabil
Ouahrani-Bettache, Safia
Drake, Kenneth L
Adams, L Garry
Köhler, Stephan
Occhialini, Alessandra
Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses
title Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses
title_full Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses
title_fullStr Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses
title_full_unstemmed Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses
title_short Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses
title_sort global rsh-dependent transcription profile of brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710219/
https://www.ncbi.nlm.nih.gov/pubmed/23834488
http://dx.doi.org/10.1186/1471-2164-14-459
work_keys_str_mv AT hannanabil globalrshdependenttranscriptionprofileofbrucellasuisduringstringentresponseunravelsadaptationtonutrientstarvationandcrosstalkwithotherstressresponses
AT ouahranibettachesafia globalrshdependenttranscriptionprofileofbrucellasuisduringstringentresponseunravelsadaptationtonutrientstarvationandcrosstalkwithotherstressresponses
AT drakekennethl globalrshdependenttranscriptionprofileofbrucellasuisduringstringentresponseunravelsadaptationtonutrientstarvationandcrosstalkwithotherstressresponses
AT adamslgarry globalrshdependenttranscriptionprofileofbrucellasuisduringstringentresponseunravelsadaptationtonutrientstarvationandcrosstalkwithotherstressresponses
AT kohlerstephan globalrshdependenttranscriptionprofileofbrucellasuisduringstringentresponseunravelsadaptationtonutrientstarvationandcrosstalkwithotherstressresponses
AT occhialinialessandra globalrshdependenttranscriptionprofileofbrucellasuisduringstringentresponseunravelsadaptationtonutrientstarvationandcrosstalkwithotherstressresponses