Cargando…
RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction
BACKGROUND: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement. However, the RNA-seq sequence data can be biased during library constructions...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710281/ https://www.ncbi.nlm.nih.gov/pubmed/23826734 http://dx.doi.org/10.1186/1745-6150-8-16 |