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RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction
BACKGROUND: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement. However, the RNA-seq sequence data can be biased during library constructions...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710281/ https://www.ncbi.nlm.nih.gov/pubmed/23826734 http://dx.doi.org/10.1186/1745-6150-8-16 |