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Efficiency of puromycin-based technologies mediated by release factors and a ribosome recycling factor

Two puromycin-based techniques, in vitro virus (IVV) and C-terminal labelling of proteins, were developed based on the observation that puromycin binds the C-terminus of a protein. Puromycin technology is a useful tool for the detection of proteins and analysis of protein–protein interactions (PPIs)...

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Detalles Bibliográficos
Autores principales: Ohashi, Hiroyuki, Ishizaka, Masamichi, Hirai, Naoya, Miyamoto-Sato, Etsuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711395/
https://www.ncbi.nlm.nih.gov/pubmed/23824411
http://dx.doi.org/10.1093/protein/gzt031