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Efficiency of puromycin-based technologies mediated by release factors and a ribosome recycling factor
Two puromycin-based techniques, in vitro virus (IVV) and C-terminal labelling of proteins, were developed based on the observation that puromycin binds the C-terminus of a protein. Puromycin technology is a useful tool for the detection of proteins and analysis of protein–protein interactions (PPIs)...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711395/ https://www.ncbi.nlm.nih.gov/pubmed/23824411 http://dx.doi.org/10.1093/protein/gzt031 |