Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks

An improved method for genetic transformation of cucumber (Cucumis sativus L. cv. Shinhokusei No. 1) was developed. Vacuum infiltration of cotyledonary explants with Agrobacterium suspension enhanced the efficiency of Agrobacterium infection in the proximal regions of explants. Co-cultivation on fil...

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Detalles Bibliográficos
Autores principales: Nanasato, Yoshihiko, Konagaya, Ken-ichi, Okuzaki, Ayako, Tsuda, Mai, Tabei, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Japan 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712137/
https://www.ncbi.nlm.nih.gov/pubmed/23874354
http://dx.doi.org/10.1007/s11816-012-0260-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712137/
https://www.ncbi.nlm.nih.gov/pubmed/23874354
http://dx.doi.org/10.1007/s11816-012-0260-1

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