827Spatio-Temporal Quantification of FRET in Living Cells by Fast Time-Domain FLIM: A Comparative Study of Non-Fitting Methods

Förster Resonance Energy Transfer (FRET) measured with Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique to investigate spatio-temporal regulation of protein-protein interactions in living cells. When using standard fitting methods to analyze time domain FLIM, the correct estim...

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Detalles Bibliográficos
Autores principales: Leray, Aymeric, Padilla-Parra, Sergi, Roul, Julien, Héliot, Laurent, Tramier, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3715500/
https://www.ncbi.nlm.nih.gov/pubmed/23874948
http://dx.doi.org/10.1371/journal.pone.0069335

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3715500/
https://www.ncbi.nlm.nih.gov/pubmed/23874948
http://dx.doi.org/10.1371/journal.pone.0069335

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