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Indeterminate tcdB using a Clostridium difficile PCR assay: a retrospective cohort study

BACKGROUND: C. difficile (CD) real-time polymerase chain reaction (PCR) for toxin B gene (tcdB) is more sensitive, and reduces turnaround time when compared to toxin immunoassay. We noted typical amplification curves with high tcdB cycle thresholds (Ct) and low endpoints (Ept) that are labeled negat...

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Detalles Bibliográficos
Autores principales: Leis, Jerome A, Gold, Wayne L, Ng, John, Hirji, Zahir, Pillai, Dylan R, Broukhanski, George, Raggiunti, Paula, Hota, Susy, McGeer, Allison, Poutanen, Susan M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718660/
https://www.ncbi.nlm.nih.gov/pubmed/23865713
http://dx.doi.org/10.1186/1471-2334-13-324