Synergistic and tunable human gene activation by combinations of synthetic transcription factors

Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of...

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Autores principales: Perez-Pinera, Pablo, Ousterout, David G., Brunger, Jonathan M., Farin, Alicia M., Glass, Katherine A., Guilak, Farshid, Crawford, Gregory E., Hartemink, Alexander J., Gersbach, Charles A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719416/
https://www.ncbi.nlm.nih.gov/pubmed/23377379
http://dx.doi.org/10.1038/nmeth.2361
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author Perez-Pinera, Pablo
Ousterout, David G.
Brunger, Jonathan M.
Farin, Alicia M.
Glass, Katherine A.
Guilak, Farshid
Crawford, Gregory E.
Hartemink, Alexander J.
Gersbach, Charles A.
author_facet Perez-Pinera, Pablo
Ousterout, David G.
Brunger, Jonathan M.
Farin, Alicia M.
Glass, Katherine A.
Guilak, Farshid
Crawford, Gregory E.
Hartemink, Alexander J.
Gersbach, Charles A.
author_sort Perez-Pinera, Pablo
collection PubMed
description Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator–like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology.
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spelling pubmed-37194162013-09-01 Synergistic and tunable human gene activation by combinations of synthetic transcription factors Perez-Pinera, Pablo Ousterout, David G. Brunger, Jonathan M. Farin, Alicia M. Glass, Katherine A. Guilak, Farshid Crawford, Gregory E. Hartemink, Alexander J. Gersbach, Charles A. Nat Methods Article Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator–like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology. 2013-02-03 2013-03 /pmc/articles/PMC3719416/ /pubmed/23377379 http://dx.doi.org/10.1038/nmeth.2361 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Perez-Pinera, Pablo
Ousterout, David G.
Brunger, Jonathan M.
Farin, Alicia M.
Glass, Katherine A.
Guilak, Farshid
Crawford, Gregory E.
Hartemink, Alexander J.
Gersbach, Charles A.
Synergistic and tunable human gene activation by combinations of synthetic transcription factors
title Synergistic and tunable human gene activation by combinations of synthetic transcription factors
title_full Synergistic and tunable human gene activation by combinations of synthetic transcription factors
title_fullStr Synergistic and tunable human gene activation by combinations of synthetic transcription factors
title_full_unstemmed Synergistic and tunable human gene activation by combinations of synthetic transcription factors
title_short Synergistic and tunable human gene activation by combinations of synthetic transcription factors
title_sort synergistic and tunable human gene activation by combinations of synthetic transcription factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719416/
https://www.ncbi.nlm.nih.gov/pubmed/23377379
http://dx.doi.org/10.1038/nmeth.2361
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