Improved Bacterial Mutagenesis by High-Frequency Allele Exchange, Demonstrated in Clostridium difficile and Streptococcus suis

Here we show that the frequency of mutant isolation by two-step allele exchange can be improved by increasing the length of homologous DNA and the opportunity for recombination, obviating the need for counterselection markers. These principles are demonstrated in Clostridium difficile and Streptococ...

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Detalles Bibliográficos
Autores principales: Faulds-Pain, Alexandra, Wren, Brendan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719504/
https://www.ncbi.nlm.nih.gov/pubmed/23728809
http://dx.doi.org/10.1128/AEM.01195-13

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3719504/
https://www.ncbi.nlm.nih.gov/pubmed/23728809
http://dx.doi.org/10.1128/AEM.01195-13

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