Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes

Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to...

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Detalles Bibliográficos
Autores principales: Shin, Seung Chul, Ahn, Do Hwan, Kim, Su Jin, Lee, Hyoungseok, Oh, Tae-Jin, Lee, Jong Eun, Park, Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720884/
https://www.ncbi.nlm.nih.gov/pubmed/23894349
http://dx.doi.org/10.1371/journal.pone.0068824
Descripción
Sumario:Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.

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