Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes

Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to...

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Detalles Bibliográficos
Autores principales: Shin, Seung Chul, Ahn, Do Hwan, Kim, Su Jin, Lee, Hyoungseok, Oh, Tae-Jin, Lee, Jong Eun, Park, Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720884/
https://www.ncbi.nlm.nih.gov/pubmed/23894349
http://dx.doi.org/10.1371/journal.pone.0068824
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author Shin, Seung Chul
Ahn, Do Hwan
Kim, Su Jin
Lee, Hyoungseok
Oh, Tae-Jin
Lee, Jong Eun
Park, Hyun
author_facet Shin, Seung Chul
Ahn, Do Hwan
Kim, Su Jin
Lee, Hyoungseok
Oh, Tae-Jin
Lee, Jong Eun
Park, Hyun
author_sort Shin, Seung Chul
collection PubMed
description Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.
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spelling pubmed-37208842013-07-26 Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes Shin, Seung Chul Ahn, Do Hwan Kim, Su Jin Lee, Hyoungseok Oh, Tae-Jin Lee, Jong Eun Park, Hyun PLoS One Research Article Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution. Public Library of Science 2013-07-23 /pmc/articles/PMC3720884/ /pubmed/23894349 http://dx.doi.org/10.1371/journal.pone.0068824 Text en © 2013 Shin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shin, Seung Chul
Ahn, Do Hwan
Kim, Su Jin
Lee, Hyoungseok
Oh, Tae-Jin
Lee, Jong Eun
Park, Hyun
Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes
title Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes
title_full Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes
title_fullStr Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes
title_full_unstemmed Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes
title_short Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes
title_sort advantages of single-molecule real-time sequencing in high-gc content genomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720884/
https://www.ncbi.nlm.nih.gov/pubmed/23894349
http://dx.doi.org/10.1371/journal.pone.0068824
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