PCR-Induced Transitions Are the Major Source of Error in Cleaned Ultra-Deep Pyrosequencing Data

BACKGROUND: Ultra-deep pyrosequencing (UDPS) is used to identify rare sequence variants. The sequence depth is influenced by several factors including the error frequency of PCR and UDPS. This study investigated the characteristics and source of errors in raw and cleaned UDPS data. RESULTS: UDPS of...

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Detalles Bibliográficos
Autores principales: Brodin, Johanna, Mild, Mattias, Hedskog, Charlotte, Sherwood, Ellen, Leitner, Thomas, Andersson, Björn, Albert, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720931/
https://www.ncbi.nlm.nih.gov/pubmed/23894647
http://dx.doi.org/10.1371/journal.pone.0070388

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720931/
https://www.ncbi.nlm.nih.gov/pubmed/23894647
http://dx.doi.org/10.1371/journal.pone.0070388

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