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Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients
Despite the growing understanding of the mechanisms of carcinogenesis, cancers of the central nervous system are usually associated with unfavorable prognosis. The use of an appropriate molecular marker may improve the treatment outcome by allowing early diagnosis and treatment susceptibility monito...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720989/ https://www.ncbi.nlm.nih.gov/pubmed/23661397 http://dx.doi.org/10.1007/s13353-013-0149-x |
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author | Majchrzak-Celińska, Aleksandra Paluszczak, Jarosław Kleszcz, Robert Magiera, Marta Barciszewska, Anna-Maria Nowak, Stanisław Baer-Dubowska, Wanda |
author_facet | Majchrzak-Celińska, Aleksandra Paluszczak, Jarosław Kleszcz, Robert Magiera, Marta Barciszewska, Anna-Maria Nowak, Stanisław Baer-Dubowska, Wanda |
author_sort | Majchrzak-Celińska, Aleksandra |
collection | PubMed |
description | Despite the growing understanding of the mechanisms of carcinogenesis, cancers of the central nervous system are usually associated with unfavorable prognosis. The use of an appropriate molecular marker may improve the treatment outcome by allowing early diagnosis and treatment susceptibility monitoring. Since methylation of tumor-derived DNA can be detected in the serum of cancer patients, this makes DNA methylation-based biomarkers one of the most promising diagnostic strategies. In this study, the methylation profiles of MGMT, RASSF1A, p15INK4B, and p14ARF genes were evaluated in serum free-circulating DNA and the corresponding tumor tissue in a group of 33 primary or metastatic central nervous system cancer patients. Gene promoter methylation was assessed using methylation-specific polymerase chain reaction (PCR). All the tested genes were found to be methylated to a different extent in both serum and tumor samples. In comparison to metastatic brain tumor patients, the patients with glial tumors were characterized by a higher frequency of gene hypermethylation. The hypermethylation of RASSF1A differentiated primary from metastatic brain cancers. Moreover, the gene methylation profiles observed in serum, in most cases, matched the methylation profiles detected in paired tumor samples. |
format | Online Article Text |
id | pubmed-3720989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-37209892013-07-31 Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients Majchrzak-Celińska, Aleksandra Paluszczak, Jarosław Kleszcz, Robert Magiera, Marta Barciszewska, Anna-Maria Nowak, Stanisław Baer-Dubowska, Wanda J Appl Genet Human Genetics • Original Paper Despite the growing understanding of the mechanisms of carcinogenesis, cancers of the central nervous system are usually associated with unfavorable prognosis. The use of an appropriate molecular marker may improve the treatment outcome by allowing early diagnosis and treatment susceptibility monitoring. Since methylation of tumor-derived DNA can be detected in the serum of cancer patients, this makes DNA methylation-based biomarkers one of the most promising diagnostic strategies. In this study, the methylation profiles of MGMT, RASSF1A, p15INK4B, and p14ARF genes were evaluated in serum free-circulating DNA and the corresponding tumor tissue in a group of 33 primary or metastatic central nervous system cancer patients. Gene promoter methylation was assessed using methylation-specific polymerase chain reaction (PCR). All the tested genes were found to be methylated to a different extent in both serum and tumor samples. In comparison to metastatic brain tumor patients, the patients with glial tumors were characterized by a higher frequency of gene hypermethylation. The hypermethylation of RASSF1A differentiated primary from metastatic brain cancers. Moreover, the gene methylation profiles observed in serum, in most cases, matched the methylation profiles detected in paired tumor samples. Springer Berlin Heidelberg 2013-05-10 2013 /pmc/articles/PMC3720989/ /pubmed/23661397 http://dx.doi.org/10.1007/s13353-013-0149-x Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Human Genetics • Original Paper Majchrzak-Celińska, Aleksandra Paluszczak, Jarosław Kleszcz, Robert Magiera, Marta Barciszewska, Anna-Maria Nowak, Stanisław Baer-Dubowska, Wanda Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients |
title | Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients |
title_full | Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients |
title_fullStr | Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients |
title_full_unstemmed | Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients |
title_short | Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients |
title_sort | detection of mgmt, rassf1a, p15ink4b, and p14arf promoter methylation in circulating tumor-derived dna of central nervous system cancer patients |
topic | Human Genetics • Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720989/ https://www.ncbi.nlm.nih.gov/pubmed/23661397 http://dx.doi.org/10.1007/s13353-013-0149-x |
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