Enhanced plasmid production in miniaturized high-cell-density cultures of Escherichia coli supported with perfluorinated oxygen carrier

A simple method for plasmid minipreps in closed 1.5 mL microcentrifuge tubes using a cultivation medium with internal substrate delivery (EnBase(®)) in combination with a two-phase perfluorodecalin (PFD) system supplying additional oxygen to the E. coli culture is described. The procedure can simply...

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Detalles Bibliográficos
Autores principales: Pilarek, Maciej, Brand, Eva, Hillig, Friederike, Krause, Mirja, Neubauer, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2012
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722437/
https://www.ncbi.nlm.nih.gov/pubmed/23178986
http://dx.doi.org/10.1007/s00449-012-0861-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722437/
https://www.ncbi.nlm.nih.gov/pubmed/23178986
http://dx.doi.org/10.1007/s00449-012-0861-7

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