The splicing regulator PTBP2 is an AID interacting protein and promotes binding of AID to switch region DNA

During immunoglobulin class switch recombination (CSR), activation induced cytidine deaminase (AID) induces DNA double strand breaks into transcribed, repetitive DNA elements called switch sequences. The mechanism that promotes the binding of AID specifically to switch regions remains to be elucidat...

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Detalles Bibliográficos
Autores principales: Nowak, Urszula, Matthews, Allysia, Zheng, Simin, Chaudhuri, Jayanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724472/
https://www.ncbi.nlm.nih.gov/pubmed/21186367
http://dx.doi.org/10.1038/ni.1977
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author Nowak, Urszula
Matthews, Allysia
Zheng, Simin
Chaudhuri, Jayanta
author_facet Nowak, Urszula
Matthews, Allysia
Zheng, Simin
Chaudhuri, Jayanta
author_sort Nowak, Urszula
collection PubMed
description During immunoglobulin class switch recombination (CSR), activation induced cytidine deaminase (AID) induces DNA double strand breaks into transcribed, repetitive DNA elements called switch sequences. The mechanism that promotes the binding of AID specifically to switch regions remains to be elucidated. We have used a proteomic screen that employs in vivo biotinylation of AID and have identified the splicing regulator polypyrimidine tract binding protein-2 (PTBP2) as an AID interactor. Short hairpin RNA-mediated knock-down of PTBP2 in B cells led to a striking reduction in binding of AID to transcribed switch regions that resulted in marked impairment of CSR. PTBP2 is thus an effector of CSR that promotes binding of AID to switch region DNA.
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spelling pubmed-37244722013-07-26 The splicing regulator PTBP2 is an AID interacting protein and promotes binding of AID to switch region DNA Nowak, Urszula Matthews, Allysia Zheng, Simin Chaudhuri, Jayanta Nat Immunol Article During immunoglobulin class switch recombination (CSR), activation induced cytidine deaminase (AID) induces DNA double strand breaks into transcribed, repetitive DNA elements called switch sequences. The mechanism that promotes the binding of AID specifically to switch regions remains to be elucidated. We have used a proteomic screen that employs in vivo biotinylation of AID and have identified the splicing regulator polypyrimidine tract binding protein-2 (PTBP2) as an AID interactor. Short hairpin RNA-mediated knock-down of PTBP2 in B cells led to a striking reduction in binding of AID to transcribed switch regions that resulted in marked impairment of CSR. PTBP2 is thus an effector of CSR that promotes binding of AID to switch region DNA. 2010-12-26 2011-02 /pmc/articles/PMC3724472/ /pubmed/21186367 http://dx.doi.org/10.1038/ni.1977 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Nowak, Urszula
Matthews, Allysia
Zheng, Simin
Chaudhuri, Jayanta
The splicing regulator PTBP2 is an AID interacting protein and promotes binding of AID to switch region DNA
title The splicing regulator PTBP2 is an AID interacting protein and promotes binding of AID to switch region DNA
title_full The splicing regulator PTBP2 is an AID interacting protein and promotes binding of AID to switch region DNA
title_fullStr The splicing regulator PTBP2 is an AID interacting protein and promotes binding of AID to switch region DNA
title_full_unstemmed The splicing regulator PTBP2 is an AID interacting protein and promotes binding of AID to switch region DNA
title_short The splicing regulator PTBP2 is an AID interacting protein and promotes binding of AID to switch region DNA
title_sort splicing regulator ptbp2 is an aid interacting protein and promotes binding of aid to switch region dna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724472/
https://www.ncbi.nlm.nih.gov/pubmed/21186367
http://dx.doi.org/10.1038/ni.1977
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