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Lipid, Detergent, and Coomassie Blue G-250 Affect the Migration of Small Membrane Proteins in Blue Native Gels: MITOCHONDRIAL CARRIERS MIGRATE AS MONOMERS NOT DIMERS

Blue native gel electrophoresis is a popular method for the determination of the oligomeric state of membrane proteins. Studies using this technique have reported that mitochondrial carriers are dimeric (composed of two ∼32-kDa monomers) and, in some cases, can form physiologically relevant associat...

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Detalles Bibliográficos
Autores principales: Crichton, Paul G., Harding, Marilyn, Ruprecht, Jonathan J., Lee, Yang, Kunji, Edmund R. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724668/
https://www.ncbi.nlm.nih.gov/pubmed/23744064
http://dx.doi.org/10.1074/jbc.M113.484329