A Unique Mitochondrial Transcription Factor B Protein in Dictyostelium discoideum

Unlike their bacteriophage homologs, mitochondrial RNA polymerases require the assistance of transcription factors in order to transcribe mitochondrial DNA efficiently. The transcription factor A family has been shown to be important for transcription of the human mitochondrial DNA, with some of its...

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Autores principales: Manna, Sam, Le, Phuong, Barth, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724811/
https://www.ncbi.nlm.nih.gov/pubmed/23923009
http://dx.doi.org/10.1371/journal.pone.0070614
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author Manna, Sam
Le, Phuong
Barth, Christian
author_facet Manna, Sam
Le, Phuong
Barth, Christian
author_sort Manna, Sam
collection PubMed
description Unlike their bacteriophage homologs, mitochondrial RNA polymerases require the assistance of transcription factors in order to transcribe mitochondrial DNA efficiently. The transcription factor A family has been shown to be important for transcription of the human mitochondrial DNA, with some of its regulatory activity located in its extended C-terminal tail. The mitochondrial transcription factor B family often has functions not only in transcription, but also in mitochondrial rRNA modification, a hallmark of its α-proteobacterial origin. We have identified and characterised a mitochondrial transcription factor B homolog in the soil dwelling cellular slime mould Dictyostelium discoideum, an organism widely established as a model for studying eukaryotic cell biology. Using in bacterio functional assays, we demonstrate that the mitochondrial transcription factor B homolog not only functions as a mitochondrial transcription factor, but that it also has a role in rRNA methylation. Additionally, we show that the transcriptional activation properties of the D. discoideum protein are located in its extended C-terminal tail, a feature not seen before in the mitochondrial transcription factor B family, but reminiscent of the human mitochondrial transcription factor A. This report contributes to our current understanding of the complexities of mitochondrial transcription, and its evolution in eukaryotes.
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spelling pubmed-37248112013-08-06 A Unique Mitochondrial Transcription Factor B Protein in Dictyostelium discoideum Manna, Sam Le, Phuong Barth, Christian PLoS One Research Article Unlike their bacteriophage homologs, mitochondrial RNA polymerases require the assistance of transcription factors in order to transcribe mitochondrial DNA efficiently. The transcription factor A family has been shown to be important for transcription of the human mitochondrial DNA, with some of its regulatory activity located in its extended C-terminal tail. The mitochondrial transcription factor B family often has functions not only in transcription, but also in mitochondrial rRNA modification, a hallmark of its α-proteobacterial origin. We have identified and characterised a mitochondrial transcription factor B homolog in the soil dwelling cellular slime mould Dictyostelium discoideum, an organism widely established as a model for studying eukaryotic cell biology. Using in bacterio functional assays, we demonstrate that the mitochondrial transcription factor B homolog not only functions as a mitochondrial transcription factor, but that it also has a role in rRNA methylation. Additionally, we show that the transcriptional activation properties of the D. discoideum protein are located in its extended C-terminal tail, a feature not seen before in the mitochondrial transcription factor B family, but reminiscent of the human mitochondrial transcription factor A. This report contributes to our current understanding of the complexities of mitochondrial transcription, and its evolution in eukaryotes. Public Library of Science 2013-07-26 /pmc/articles/PMC3724811/ /pubmed/23923009 http://dx.doi.org/10.1371/journal.pone.0070614 Text en © 2013 Manna et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Manna, Sam
Le, Phuong
Barth, Christian
A Unique Mitochondrial Transcription Factor B Protein in Dictyostelium discoideum
title A Unique Mitochondrial Transcription Factor B Protein in Dictyostelium discoideum
title_full A Unique Mitochondrial Transcription Factor B Protein in Dictyostelium discoideum
title_fullStr A Unique Mitochondrial Transcription Factor B Protein in Dictyostelium discoideum
title_full_unstemmed A Unique Mitochondrial Transcription Factor B Protein in Dictyostelium discoideum
title_short A Unique Mitochondrial Transcription Factor B Protein in Dictyostelium discoideum
title_sort unique mitochondrial transcription factor b protein in dictyostelium discoideum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724811/
https://www.ncbi.nlm.nih.gov/pubmed/23923009
http://dx.doi.org/10.1371/journal.pone.0070614
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