Removing PCR for the elimination of undesired DNA fragments cycle by cycle

A novel removing polymerase chain reaction (R-PCR) technique was developed, which can eliminate undesired genes, cycle by cycle, with efficiencies of 60.9% (cDNAs), 73.6% (genomic DNAs), and ~ 100% (four DNA fragments were tested). Major components of the R-PCR include drivers, a thermostable restri...

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Detalles Bibliográficos
Autores principales: Huan, Jiaojiao, Wan, Kangkang, Liu, Yunjun, Dong, Wubei, Wang, Guoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725479/
https://www.ncbi.nlm.nih.gov/pubmed/23892515
http://dx.doi.org/10.1038/srep02303

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725479/
https://www.ncbi.nlm.nih.gov/pubmed/23892515
http://dx.doi.org/10.1038/srep02303

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