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Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro
Programmed death-1 (PD-1) is a known immunoinhibitory receptor that contributes to immune evasion of various tumor cells and pathogens causing chronic infection, such as bovine leukemia virus (BLV) infection. First, in this study, to establish a method for the expression and functional analysis of b...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726328/ https://www.ncbi.nlm.nih.gov/pubmed/23876077 http://dx.doi.org/10.1186/1297-9716-44-59 |
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author | Ikebuchi, Ryoyo Konnai, Satoru Okagawa, Tomohiro Yokoyama, Kazumasa Nakajima, Chie Suzuki, Yasuhiko Murata, Shiro Ohashi, Kazuhiko |
author_facet | Ikebuchi, Ryoyo Konnai, Satoru Okagawa, Tomohiro Yokoyama, Kazumasa Nakajima, Chie Suzuki, Yasuhiko Murata, Shiro Ohashi, Kazuhiko |
author_sort | Ikebuchi, Ryoyo |
collection | PubMed |
description | Programmed death-1 (PD-1) is a known immunoinhibitory receptor that contributes to immune evasion of various tumor cells and pathogens causing chronic infection, such as bovine leukemia virus (BLV) infection. First, in this study, to establish a method for the expression and functional analysis of bovine PD-1, hybridomas producing monoclonal antibodies (mAb) specific for bovine PD-1 were established. Treatment with these anti-PD-1 mAb enhanced interferon-gamma (IFN-γ) production of bovine peripheral blood mononuclear cells (PBMC). Next, to examine whether PD-1 blockade by anti-PD-1 mAb could upregulate the immune reaction during chronic infection, the expression and functional analysis of PD-1 in PBMC isolated from BLV-infected cattle with or without lymphoma were performed using anti-PD-1 mAb. The frequencies of both PD-1(+) CD4(+) T cells in blood and lymph node and PD-1(+) CD8(+) T cells in lymph node were higher in BLV-infected cattle with lymphoma than those without lymphoma or control uninfected cattle. PD-1 blockade enhanced IFN-γ production and proliferation and reduced BLV-gp51 expression and B-cell activation in PBMC from BLV-infected cattle in response to BLV-gp51 peptide mixture. These data show that anti-bovine PD-1 mAb could provide a new therapy to control BLV infection via upregulation of immune response. |
format | Online Article Text |
id | pubmed-3726328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-37263282013-07-30 Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro Ikebuchi, Ryoyo Konnai, Satoru Okagawa, Tomohiro Yokoyama, Kazumasa Nakajima, Chie Suzuki, Yasuhiko Murata, Shiro Ohashi, Kazuhiko Vet Res Research Programmed death-1 (PD-1) is a known immunoinhibitory receptor that contributes to immune evasion of various tumor cells and pathogens causing chronic infection, such as bovine leukemia virus (BLV) infection. First, in this study, to establish a method for the expression and functional analysis of bovine PD-1, hybridomas producing monoclonal antibodies (mAb) specific for bovine PD-1 were established. Treatment with these anti-PD-1 mAb enhanced interferon-gamma (IFN-γ) production of bovine peripheral blood mononuclear cells (PBMC). Next, to examine whether PD-1 blockade by anti-PD-1 mAb could upregulate the immune reaction during chronic infection, the expression and functional analysis of PD-1 in PBMC isolated from BLV-infected cattle with or without lymphoma were performed using anti-PD-1 mAb. The frequencies of both PD-1(+) CD4(+) T cells in blood and lymph node and PD-1(+) CD8(+) T cells in lymph node were higher in BLV-infected cattle with lymphoma than those without lymphoma or control uninfected cattle. PD-1 blockade enhanced IFN-γ production and proliferation and reduced BLV-gp51 expression and B-cell activation in PBMC from BLV-infected cattle in response to BLV-gp51 peptide mixture. These data show that anti-bovine PD-1 mAb could provide a new therapy to control BLV infection via upregulation of immune response. BioMed Central 2013 2013-07-22 /pmc/articles/PMC3726328/ /pubmed/23876077 http://dx.doi.org/10.1186/1297-9716-44-59 Text en Copyright © 2013 Ikebuchi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Ikebuchi, Ryoyo Konnai, Satoru Okagawa, Tomohiro Yokoyama, Kazumasa Nakajima, Chie Suzuki, Yasuhiko Murata, Shiro Ohashi, Kazuhiko Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro |
title | Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro |
title_full | Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro |
title_fullStr | Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro |
title_full_unstemmed | Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro |
title_short | Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro |
title_sort | blockade of bovine pd-1 increases t cell function and inhibits bovine leukemia virus expression in b cells in vitro |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726328/ https://www.ncbi.nlm.nih.gov/pubmed/23876077 http://dx.doi.org/10.1186/1297-9716-44-59 |
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