Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

The Fluorescence in situ Hybridization (FISH) method allows one to detect nucleic acids in the native cellular environment. Here we provide a protocol for using FISH to quantify the number of mRNAs in single yeast cells. Cells can be grown in any condition of interest and then fixed and made permeab...

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Detalles Bibliográficos
Autores principales: McIsaac, R. Scott, Silverman, Sanford J., Parsons, Lance, Xu, Ping, Briehof, Ryan, McClean, Megan N., Botstein, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2013
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3727456/
https://www.ncbi.nlm.nih.gov/pubmed/23793137
http://dx.doi.org/10.3791/50382

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3727456/
https://www.ncbi.nlm.nih.gov/pubmed/23793137
http://dx.doi.org/10.3791/50382

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