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Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families

In this study, a protocol is described for rapid preparation of an enriched, reasonably pure fraction of nuclear proteins from the leaves of tobacco (Nicotiana tabacum), potato (Solanum tuberosum) and apple (Malus domestica). The protocol gives reproducible results and can be carried out quickly in...

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Detalles Bibliográficos
Autores principales: Sikorskaite, Sidona, Rajamäki, Minna-Liisa, Baniulis, Danas, Stanys, Vidmantas, Valkonen, Jari PT
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728069/
https://www.ncbi.nlm.nih.gov/pubmed/23886449
http://dx.doi.org/10.1186/1746-4811-9-31