Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS

Concentrations of l-tryptophan (l-Trp) and its metabolite, l-kynurenine (l-KYN), in sera of 19 normal subjects (age: 23.6 ± 3.5 y, male: 8, female: 11) were determined by high-performance liquid chromatography with mass-spectrometric detection, following their derivatization with (R)-(−)-4-(N, N-dim...

Descripción completa

Detalles Bibliográficos
Autores principales: Ohashi, Hayato, Iizuka, Hideaki, Yoshihara, Shunsuke, Otani, Hayato, Kume, Misato, Sadamoto, Kiyomi, Ichiba, Hideaki, Fukushima, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2013
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729339/
https://www.ncbi.nlm.nih.gov/pubmed/23922499
http://dx.doi.org/10.4137/IJTR.S11459
_version_ 1782278956235358208
author Ohashi, Hayato
Iizuka, Hideaki
Yoshihara, Shunsuke
Otani, Hayato
Kume, Misato
Sadamoto, Kiyomi
Ichiba, Hideaki
Fukushima, Takeshi
author_facet Ohashi, Hayato
Iizuka, Hideaki
Yoshihara, Shunsuke
Otani, Hayato
Kume, Misato
Sadamoto, Kiyomi
Ichiba, Hideaki
Fukushima, Takeshi
author_sort Ohashi, Hayato
collection PubMed
description Concentrations of l-tryptophan (l-Trp) and its metabolite, l-kynurenine (l-KYN), in sera of 19 normal subjects (age: 23.6 ± 3.5 y, male: 8, female: 11) were determined by high-performance liquid chromatography with mass-spectrometric detection, following their derivatization with (R)-(−)-4-(N, N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). A significant positive correlation between l-Trp and l-KYN concentrations was observed (r = 0.532, P < 0.05). Serum l-Trp concentration in male subjects (95.65 ± 4.27 μM) was significantly higher than that in female subjects (79.20 ± 3.34 μM; P < 0.05), while no significant differences in l-KYN concentration or the l-KYN:l-Trp ratio were observed between male and female subjects.
format Online
Article
Text
id pubmed-3729339
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Libertas Academica
record_format MEDLINE/PubMed
spelling pubmed-37293392013-08-06 Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS Ohashi, Hayato Iizuka, Hideaki Yoshihara, Shunsuke Otani, Hayato Kume, Misato Sadamoto, Kiyomi Ichiba, Hideaki Fukushima, Takeshi Int J Tryptophan Res Original Research Concentrations of l-tryptophan (l-Trp) and its metabolite, l-kynurenine (l-KYN), in sera of 19 normal subjects (age: 23.6 ± 3.5 y, male: 8, female: 11) were determined by high-performance liquid chromatography with mass-spectrometric detection, following their derivatization with (R)-(−)-4-(N, N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). A significant positive correlation between l-Trp and l-KYN concentrations was observed (r = 0.532, P < 0.05). Serum l-Trp concentration in male subjects (95.65 ± 4.27 μM) was significantly higher than that in female subjects (79.20 ± 3.34 μM; P < 0.05), while no significant differences in l-KYN concentration or the l-KYN:l-Trp ratio were observed between male and female subjects. Libertas Academica 2013-07-21 /pmc/articles/PMC3729339/ /pubmed/23922499 http://dx.doi.org/10.4137/IJTR.S11459 Text en © 2013 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article published under the Creative Commons CC-BY-NC 3.0 license.
spellingShingle Original Research
Ohashi, Hayato
Iizuka, Hideaki
Yoshihara, Shunsuke
Otani, Hayato
Kume, Misato
Sadamoto, Kiyomi
Ichiba, Hideaki
Fukushima, Takeshi
Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS
title Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS
title_full Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS
title_fullStr Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS
title_full_unstemmed Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS
title_short Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS
title_sort determination of l-tryptophan and l-kynurenine in human serum by using lc-ms after derivatization with (r)-dbd-pyncs
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729339/
https://www.ncbi.nlm.nih.gov/pubmed/23922499
http://dx.doi.org/10.4137/IJTR.S11459
work_keys_str_mv AT ohashihayato determinationofltryptophanandlkynurenineinhumanserumbyusinglcmsafterderivatizationwithrdbdpyncs
AT iizukahideaki determinationofltryptophanandlkynurenineinhumanserumbyusinglcmsafterderivatizationwithrdbdpyncs
AT yoshiharashunsuke determinationofltryptophanandlkynurenineinhumanserumbyusinglcmsafterderivatizationwithrdbdpyncs
AT otanihayato determinationofltryptophanandlkynurenineinhumanserumbyusinglcmsafterderivatizationwithrdbdpyncs
AT kumemisato determinationofltryptophanandlkynurenineinhumanserumbyusinglcmsafterderivatizationwithrdbdpyncs
AT sadamotokiyomi determinationofltryptophanandlkynurenineinhumanserumbyusinglcmsafterderivatizationwithrdbdpyncs
AT ichibahideaki determinationofltryptophanandlkynurenineinhumanserumbyusinglcmsafterderivatizationwithrdbdpyncs
AT fukushimatakeshi determinationofltryptophanandlkynurenineinhumanserumbyusinglcmsafterderivatizationwithrdbdpyncs

Ejemplares similares