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Comparison of first dimension IPG and NEPHGE techniques in two-dimensional gel electrophoresis experiment with cytosolic unfolded protein response in Saccharomyces cerevisiae

BACKGROUND: Two-dimensional gel electrophoresis (2DE) is one of the most popular methods in proteomics. Currently, most 2DE experiments are performed using immobilized pH gradient (IPG) in the first dimension; however, some laboratories still use carrier ampholytes-based isoelectric focusing techniq...

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Detalles Bibliográficos
Autores principales: Slibinskas, Rimantas, Ražanskas, Raimundas, Zinkevičiūtė, Rūta, Čiplys, Evaldas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729415/
https://www.ncbi.nlm.nih.gov/pubmed/23889826
http://dx.doi.org/10.1186/1477-5956-11-36