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Unusual base pairing during the decoding of a stop codon by the ribosome
During normal translation, binding of a release factor to one of the three stop codons (UGA, UAA or UAG) results in termination of protein synthesis. However, modification of the initial uridine to a pseudouridine (Ψ) allows efficient recognition and read-through of these stop codons by a transfer R...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732562/ https://www.ncbi.nlm.nih.gov/pubmed/23812587 http://dx.doi.org/10.1038/nature12302 |
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author | Fernández, Israel S. Ng, Chyan Leong Kelley, Ann C. Wu, Guowei Yu, Yi-Tao Ramakrishnan, V. |
author_facet | Fernández, Israel S. Ng, Chyan Leong Kelley, Ann C. Wu, Guowei Yu, Yi-Tao Ramakrishnan, V. |
author_sort | Fernández, Israel S. |
collection | PubMed |
description | During normal translation, binding of a release factor to one of the three stop codons (UGA, UAA or UAG) results in termination of protein synthesis. However, modification of the initial uridine to a pseudouridine (Ψ) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRNA), although it requires formation of two normally forbidden purine-purine base pairs(1). We have determined the crystal structure at 3.1 Å resolution of the 30S ribosomal subunit in complex with the anticodon stem loop of tRNA(Ser) bound to the ΨAG stop codon in the A site. The ΨA base pair at the first position is accompanied by the formation of purine-purine base pairs at the second and third positions of the codon, which display an unusual Watson-Crick/Hoogsteen geometry. The structure shows a previously unsuspected ability of the ribosomal decoding center to accommodate non-canonical base pairs. |
format | Online Article Text |
id | pubmed-3732562 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-37325622014-02-01 Unusual base pairing during the decoding of a stop codon by the ribosome Fernández, Israel S. Ng, Chyan Leong Kelley, Ann C. Wu, Guowei Yu, Yi-Tao Ramakrishnan, V. Nature Article During normal translation, binding of a release factor to one of the three stop codons (UGA, UAA or UAG) results in termination of protein synthesis. However, modification of the initial uridine to a pseudouridine (Ψ) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRNA), although it requires formation of two normally forbidden purine-purine base pairs(1). We have determined the crystal structure at 3.1 Å resolution of the 30S ribosomal subunit in complex with the anticodon stem loop of tRNA(Ser) bound to the ΨAG stop codon in the A site. The ΨA base pair at the first position is accompanied by the formation of purine-purine base pairs at the second and third positions of the codon, which display an unusual Watson-Crick/Hoogsteen geometry. The structure shows a previously unsuspected ability of the ribosomal decoding center to accommodate non-canonical base pairs. 2013-06-30 2013-08-01 /pmc/articles/PMC3732562/ /pubmed/23812587 http://dx.doi.org/10.1038/nature12302 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Fernández, Israel S. Ng, Chyan Leong Kelley, Ann C. Wu, Guowei Yu, Yi-Tao Ramakrishnan, V. Unusual base pairing during the decoding of a stop codon by the ribosome |
title | Unusual base pairing during the decoding of a stop codon by the ribosome |
title_full | Unusual base pairing during the decoding of a stop codon by the ribosome |
title_fullStr | Unusual base pairing during the decoding of a stop codon by the ribosome |
title_full_unstemmed | Unusual base pairing during the decoding of a stop codon by the ribosome |
title_short | Unusual base pairing during the decoding of a stop codon by the ribosome |
title_sort | unusual base pairing during the decoding of a stop codon by the ribosome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732562/ https://www.ncbi.nlm.nih.gov/pubmed/23812587 http://dx.doi.org/10.1038/nature12302 |
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