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Molecular interactions between the olive and the fruit fly Bactrocera oleae

BACKGROUND: The fruit fly Bactrocera oleae is the primary biotic stressor of cultivated olives, causing direct and indirect damages that significantly reduce both the yield and the quality of olive oil. To study the olive-B. oleae interaction, we conducted transcriptomic and proteomic investigations...

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Autores principales: Corrado, Giandomenico, Alagna, Fiammetta, Rocco, Mariapina, Renzone, Giovanni, Varricchio, Paola, Coppola, Valentina, Coppola, Mariangela, Garonna, Antonio, Baldoni, Luciana, Scaloni, Andrea, Rao, Rosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733423/
https://www.ncbi.nlm.nih.gov/pubmed/22694925
http://dx.doi.org/10.1186/1471-2229-12-86
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author Corrado, Giandomenico
Alagna, Fiammetta
Rocco, Mariapina
Renzone, Giovanni
Varricchio, Paola
Coppola, Valentina
Coppola, Mariangela
Garonna, Antonio
Baldoni, Luciana
Scaloni, Andrea
Rao, Rosa
author_facet Corrado, Giandomenico
Alagna, Fiammetta
Rocco, Mariapina
Renzone, Giovanni
Varricchio, Paola
Coppola, Valentina
Coppola, Mariangela
Garonna, Antonio
Baldoni, Luciana
Scaloni, Andrea
Rao, Rosa
author_sort Corrado, Giandomenico
collection PubMed
description BACKGROUND: The fruit fly Bactrocera oleae is the primary biotic stressor of cultivated olives, causing direct and indirect damages that significantly reduce both the yield and the quality of olive oil. To study the olive-B. oleae interaction, we conducted transcriptomic and proteomic investigations of the molecular response of the drupe. The identifications of genes and proteins involved in the fruit response were performed using a Suppression Subtractive Hybridisation technique and a combined bi-dimensional electrophoresis/nanoLC-ESI-LIT-MS/MS approach, respectively. RESULTS: We identified 196 ESTs and 26 protein spots as differentially expressed in olives with larval feeding tunnels. A bioinformatic analysis of the identified non-redundant EST and protein collection indicated that different molecular processes were affected, such as stress response, phytohormone signalling, transcriptional control and primary metabolism, and that a considerable proportion of the ESTs could not be classified. The altered expression of 20 transcripts was also analysed by real-time PCR, and the most striking differences were further confirmed in the fruit of a different olive variety. We also cloned the full-length coding sequences of two genes, Oe-chitinase I and Oe-PR27, and showed that these are wound-inducible genes and activated by B. oleae punctures. CONCLUSIONS: This study represents the first report that reveals the molecular players and signalling pathways involved in the interaction between the olive fruit and its most damaging biotic stressor. Drupe response is complex, involving genes and proteins involved in photosynthesis as well as in the production of ROS, the activation of different stress response pathways and the production of compounds involved in direct defence against phytophagous larvae. Among the latter, trypsin inhibitors should play a major role in drupe resistance reaction.
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spelling pubmed-37334232013-08-06 Molecular interactions between the olive and the fruit fly Bactrocera oleae Corrado, Giandomenico Alagna, Fiammetta Rocco, Mariapina Renzone, Giovanni Varricchio, Paola Coppola, Valentina Coppola, Mariangela Garonna, Antonio Baldoni, Luciana Scaloni, Andrea Rao, Rosa BMC Plant Biol Research Article BACKGROUND: The fruit fly Bactrocera oleae is the primary biotic stressor of cultivated olives, causing direct and indirect damages that significantly reduce both the yield and the quality of olive oil. To study the olive-B. oleae interaction, we conducted transcriptomic and proteomic investigations of the molecular response of the drupe. The identifications of genes and proteins involved in the fruit response were performed using a Suppression Subtractive Hybridisation technique and a combined bi-dimensional electrophoresis/nanoLC-ESI-LIT-MS/MS approach, respectively. RESULTS: We identified 196 ESTs and 26 protein spots as differentially expressed in olives with larval feeding tunnels. A bioinformatic analysis of the identified non-redundant EST and protein collection indicated that different molecular processes were affected, such as stress response, phytohormone signalling, transcriptional control and primary metabolism, and that a considerable proportion of the ESTs could not be classified. The altered expression of 20 transcripts was also analysed by real-time PCR, and the most striking differences were further confirmed in the fruit of a different olive variety. We also cloned the full-length coding sequences of two genes, Oe-chitinase I and Oe-PR27, and showed that these are wound-inducible genes and activated by B. oleae punctures. CONCLUSIONS: This study represents the first report that reveals the molecular players and signalling pathways involved in the interaction between the olive fruit and its most damaging biotic stressor. Drupe response is complex, involving genes and proteins involved in photosynthesis as well as in the production of ROS, the activation of different stress response pathways and the production of compounds involved in direct defence against phytophagous larvae. Among the latter, trypsin inhibitors should play a major role in drupe resistance reaction. BioMed Central 2012-06-13 /pmc/articles/PMC3733423/ /pubmed/22694925 http://dx.doi.org/10.1186/1471-2229-12-86 Text en Copyright © 2012 Corrado et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Corrado, Giandomenico
Alagna, Fiammetta
Rocco, Mariapina
Renzone, Giovanni
Varricchio, Paola
Coppola, Valentina
Coppola, Mariangela
Garonna, Antonio
Baldoni, Luciana
Scaloni, Andrea
Rao, Rosa
Molecular interactions between the olive and the fruit fly Bactrocera oleae
title Molecular interactions between the olive and the fruit fly Bactrocera oleae
title_full Molecular interactions between the olive and the fruit fly Bactrocera oleae
title_fullStr Molecular interactions between the olive and the fruit fly Bactrocera oleae
title_full_unstemmed Molecular interactions between the olive and the fruit fly Bactrocera oleae
title_short Molecular interactions between the olive and the fruit fly Bactrocera oleae
title_sort molecular interactions between the olive and the fruit fly bactrocera oleae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733423/
https://www.ncbi.nlm.nih.gov/pubmed/22694925
http://dx.doi.org/10.1186/1471-2229-12-86
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