Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

BACKGROUND: Fab antibody fragments in E. coli are usually directed to the oxidizing periplasmic space for correct folding. From periplasm Fab fragments may further leak into extracellular medium. Information on the cultivation parameters affecting this leakage is scarce, and the unpredictable nature...

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Detalles Bibliográficos
Autores principales: Ukkonen, Kaisa, Veijola, Johanna, Vasala, Antti, Neubauer, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733871/
https://www.ncbi.nlm.nih.gov/pubmed/23895637
http://dx.doi.org/10.1186/1475-2859-12-73

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733871/
https://www.ncbi.nlm.nih.gov/pubmed/23895637
http://dx.doi.org/10.1186/1475-2859-12-73

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