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A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay
Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogen...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC373457/ https://www.ncbi.nlm.nih.gov/pubmed/14754458 http://dx.doi.org/10.1186/1479-5876-2-4 |
| _version_ | 1782121274417348608 |
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| author | Miller, Walter J Kayton, Mark L Patton, Angela O'Connor, Sarah He, Mei Vu, Huan Baibakov, Galina Lorang, Dominique Knezevic, Vladimir Kohn, Elise Alexander, H Richard Stirling, David Payvandi, Faribourz Muller, George W Libutti, Steven K |
| author_facet | Miller, Walter J Kayton, Mark L Patton, Angela O'Connor, Sarah He, Mei Vu, Huan Baibakov, Galina Lorang, Dominique Knezevic, Vladimir Kohn, Elise Alexander, H Richard Stirling, David Payvandi, Faribourz Muller, George W Libutti, Steven K |
| author_sort | Miller, Walter J |
| collection | PubMed |
| description | Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response. |
| format | Text |
| id | pubmed-373457 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2004 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-3734572004-03-21 A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay Miller, Walter J Kayton, Mark L Patton, Angela O'Connor, Sarah He, Mei Vu, Huan Baibakov, Galina Lorang, Dominique Knezevic, Vladimir Kohn, Elise Alexander, H Richard Stirling, David Payvandi, Faribourz Muller, George W Libutti, Steven K J Transl Med Research Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response. BioMed Central 2004-01-30 /pmc/articles/PMC373457/ /pubmed/14754458 http://dx.doi.org/10.1186/1479-5876-2-4 Text en Copyright © 2004 Miller et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
| spellingShingle | Research Miller, Walter J Kayton, Mark L Patton, Angela O'Connor, Sarah He, Mei Vu, Huan Baibakov, Galina Lorang, Dominique Knezevic, Vladimir Kohn, Elise Alexander, H Richard Stirling, David Payvandi, Faribourz Muller, George W Libutti, Steven K A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay |
| title | A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay |
| title_full | A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay |
| title_fullStr | A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay |
| title_full_unstemmed | A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay |
| title_short | A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay |
| title_sort | novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (cam) assay |
| topic | Research |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC373457/ https://www.ncbi.nlm.nih.gov/pubmed/14754458 http://dx.doi.org/10.1186/1479-5876-2-4 |
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